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DL-Dithiothreitol CAS 3483-12-3 DTT

Dithiothreitol is a commonly used reducing agent, also known as dithiothreitol (DTT), which has strong reducibility. Its reducibility is largely due to the conformational stability of its oxidized hexagonal ring (containing disulfide bonds). The reducing power is affected by pH value and can only exert a reducing effect when the pH value is greater than 7. This is because only protonated thiol salt anions (- S -) have reactivity, while thiols (- SH) do not; The pKa of thiol groups is generally around 8.3.
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  • 3483-12-3

  • bosschemical

  • 3483-12-3

DL-Dithiothreitol Basic information
Product Name: DL-Dithiothreitol
CAS: 578517
MF: C4H10O2S2
MW: 154.25
EINECS: 222-468-7
Mol File: 3483-12-3.mol
DL-Dithiothreitol Chemical Properties
Melting point  41-44 °C(lit.)
Boiling point  125 °C
alpha  -0.2~+0.2°(20℃/D)(c=5,H2O)
bulk density 300kg/m3
density  1.04 g/mL at 20 °C
vapor density  5.3 (vs air)
vapor pressure  0.019-0.29Pa at 20-50℃
refractive index  1.5200 (estimate)
Fp  >230 °F
storage temp.  -20°C (or 4°C short term).
solubility  H2O: 50 mg/mL, clear, colorless
form  Powder
pka pK1:8.9 (25°C)
color  White
PH 4.0-6.0 (20-25℃, 0.1m in H2O)
Odor Unpleasant Odor
PH Range 4 - 6 at 15,4 g/l at 25 °C
biological source synthetic
Water Solubility  freely soluble
Sensitive  Air Sensitive
λmax λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100
Merck  143,376
BRN  1719757
Stability: Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C.
InChIKey VHJLVAABSRFDPM-UHFFFAOYSA-N
LogP 0.07 at 25℃ and pH5
CAS DataBase Reference 3483-12-3(CAS DataBase Reference)
NIST Chemistry Reference 2,3-Butanediol, 1,4-dimercapto-, (r*,r*)-(3483-12-3)
EPA Substance Registry System 1,4-Dithiothreitol (3483-12-3)
Absorption cut-off at 330nm

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