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IPTG/Isopropyl-beta-D-thiogalactopyranoside CAS 367-93-1

Isopropyl thiogalactoside (IPTG) is a highly effective inducer that is highly stable and not metabolized by bacteria, and is therefore widely used in laboratories. IPTG is commonly used in cloning experiments that require induction of β - galactosidase activity. It is often used in combination with X-Gal or Bluo GaChemicalbook for blue white screening of recombinant bacterial colonies that can induce the expression of lac operon in E. coli. IPTG binds to lacI repressor protein and changes its conformation to exert its effect, preventing inhibition of the β - galactosidase encoding gene lacZ.
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  • 367-93-1

IPTG Basic information
Product Name: IPTG
CAS: 367-93-1
MF: C9H18O5S
MW: 238.3
EINECS: 206-703-0
Mol File: 367-93-1.mol
IPTG Chemical Properties
Melting point  105 °C
alpha  -31 º (c=1, water)
Boiling point  350.9°C (rough estimate)
density  1.3329 (rough estimate)
refractive index  1.5060 (estimate)
storage temp.  2-8°C
solubility  Soluble in Water or Methanol.
pka 13.00±0.70(Predicted)
form  Crystalline Powder
color  White
PH 5-7 (50g/L)
biological source synthetic
Water Solubility  soluble
Merck  145,082
BRN  4631
Stability: Stable. Incompatible with strong oxidizing agents.
InChIKey BPHPUYQFMNQIOC-NXRLNHOXSA-N
CAS DataBase Reference 367-93-1(CAS DataBase Reference)
EPA Substance Registry System Isopropyl .beta.- thiogalactoside (367-93-1)

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