IPTG/Isopropyl-beta-D-thiogalactopyranoside CAS 367-93-1

Isopropyl thiogalactoside (IPTG) is a highly effective inducer that is highly stable and not metabolized by bacteria, and is therefore widely used in laboratories. IPTG is commonly used in cloning experiments that require induction of β - galactosidase activity. It is often used in combination with X-Gal or Bluo GaChemicalbook for blue white screening of recombinant bacterial colonies that can induce the expression of lac operon in E. coli. IPTG binds to lacI repressor protein and changes its conformation to exert its effect, preventing inhibition of the β - galactosidase encoding gene lacZ.