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Nitrotetrazolium blue chloride CAS 298-83-9

Nitrotetrazolium chloride (NBT, CAS 298-83-9) is a light yellow to yellow crystalline powder that is easily soluble in water and methanol. It is a commonly used chromogenic substrate in biochemistry and molecular biology, used as a substrate for dehydrogenases or in detection systems with BCIP. It generates insoluble blue/blue purple precipitates under alkaline phosphatase catalysis and is widely used in immunohistochemistry (IHC), Western blotting (WB), and in situ hybridization experiments.
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  • 298-83-9

Nitrotetrazolium blue chloride Basic information

Product Name:

Nitrotetrazolium blue chloride

CAS:

298-83-9

MF:

C40H30ClN10O6+

MW:

782.19

EINECS:

206-067-4

Mol File:

298-83-9.mol

Nitrotetrazolium blue chloride Chemical Properties

Melting point

200°C

density

1.5521 (rough estimate)

refractive index

1.7350 (estimate)

storage temp.

2-8°C

solubility

methanol: 50 mg/mL, clear, very strongly yellow

form

Powder/Solid

color

Yellow

PH

4.5-5.5

Water Solubility

Soluble in water, ethanol and methanol.

λmax

256 nm

Sensitive

Light Sensitive

BRN

4115923

Stability:

Stable, but store cold.

Biological Applications

Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting

InChIKey

FSVCQIDHPKZJSO-UHFFFAOYSA-L

SMILES

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

EPA Substance Registry System

2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)

固体.jpg
通用.jpg

Item

Standard

Result

Appearance

Yellow powder

Yellow powder

Purity

98% MIN

98.25%

Water

4.5%MAX

1.99

Solubility (5% methanol)

Yellow transparent solution

Yellow
ransparent solution

TLC

One point

Pass

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Chemical detection methods

  • Spectroscopic Analysis Methods

    Ultraviolet-Vis Spectrophotometry (UV-Vis)
    Infrared Spectroscopy (IR/FTIR)
    Atomic Absorption Spectroscopy (AAS)
    X-ray Fluorescence Spectroscopy (XRF)
    Nuclear Magnetic Resonance Spectroscopy (NMR)

  • Chromatographic Analysis

    Gas Chromatography (GC)
    High Performance Liquid Chromatography (HPLC)
    Ion Chromatography (IC)
    Thin Layer Chromatography (TLC)

  • Mass Spectrometry Analysis

    GC-MS / LC-MS/MS: Qualitative and Quantitative Analysis
    ICP-MS: Trace Metal Elements (ppb Level)
    TOF-MS: Precise Molecular Weight Determination

  • Electrochemical analysis methods
    Polypotentiometric methods
    Conductivity methods
    Polarographic methods / Voltammetric methods
    Coulometric analysis
  • Thermal Analysis Methods
    Thermogravimetric Analysis (TGA)
    Differential Scanning Calorimetry (DSC)
    Differential Thermal Analysis (DTA)
  • Classical wet chemical analysis
    Titration analysis
    Gravity analysis
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