E-mail: sales001@bosschemical.com                                              JINAN BOSS CHEM CO.,LTD
Telephone: +86-15628782329
You are here: Home » Products » Organic Chemicals » Nitrotetrazolium blue chloride CAS 298-83-9

Nitrotetrazolium blue chloride CAS 298-83-9

Nitrotetrazolium chloride (NBT, CAS 298-83-9) is a light yellow to yellow crystalline powder that is easily soluble in water and methanol. It is a commonly used chromogenic substrate in biochemistry and molecular biology, used as a substrate for dehydrogenases or in detection systems with BCIP. It generates insoluble blue/blue purple precipitates under alkaline phosphatase catalysis and is widely used in immunohistochemistry (IHC), Western blotting (WB), and in situ hybridization experiments.
Availability:

PDF Export

facebook sharing button
wechat sharing button
linkedin sharing button
whatsapp sharing button
sharethis sharing button
  • 298-83-9

  • bosschemical

  • 298-83-9

Nitrotetrazolium blue chloride Basic information

Product Name:

Nitrotetrazolium blue chloride

CAS:

298-83-9

MF:

C40H30ClN10O6+

MW:

782.19

EINECS:

206-067-4

Mol File:

298-83-9.mol

Nitrotetrazolium blue chloride Chemical Properties

Melting point

200°C

density

1.5521 (rough estimate)

refractive index

1.7350 (estimate)

storage temp.

2-8°C

solubility

methanol: 50 mg/mL, clear, very strongly yellow

form

Powder/Solid

color

Yellow

PH

4.5-5.5

Water Solubility

Soluble in water, ethanol and methanol.

λmax

256 nm

Sensitive

Light Sensitive

BRN

4115923

Stability:

Stable, but store cold.

Biological Applications

Diagnosis of Alzheimer’s disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides,yeast; generating and detecting

InChIKey

FSVCQIDHPKZJSO-UHFFFAOYSA-L

SMILES

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

EPA Substance Registry System

2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride (298-83-9)

固体.jpg
通用.jpg

Item

Standard

Result

Appearance

Yellow powder

Yellow powder

Purity

98% MIN

98.25%

Water

4.5%MAX

1.99

Solubility (5% methanol)

Yellow transparent solution

Yellow
ransparent solution

TLC

One point

Pass

Previous: 
Next: 
ABOUT COMPANY
Founded in 2010, JINAN BOSS CHEM CO.,LTD. is a large-scale hi-tech enterprise focused on the research and development of fine chemicals and international trade.​​​​​​​

Copryright 2025 JINAN BOSS CHEM CO.,LTD Support By Leadong/ Privacy Policy