Fluorescein Cas 2321-07-5

Fluorescein appears as orange yellow or light orange red crystalline powder. Melting point 314-316 ℃ (decomposition). Soluble in hot ethanol, glacial acetic acid, and alkaline solutions, exhibiting green fluorescence, insoluble in water, benzene, chloroform, and ether.

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Model:
2321-07-5
Brand:
bosschemical
Code:
2321-07-5

Product Description

Fluorescein Basic information
Product Name:	Fluorescein
CAS:	2321-07-5
MF:	C20H12O5
MW:	332.31
EINECS:	219-031-8
Mol File:	2321-07-5.mol
Fluorescein Chemical Properties
Melting point	320 °C(lit.)
Boiling point	429.44°C (rough estimate)
density	1.2739 (rough estimate)
refractive index	1.5000 (estimate)
storage temp.	room temp
solubility	Solubility Insoluble in water, ether, benzene, chloroform; soluble in ethanol, methanol, acetone, Iethyl acetate, N,N-dimethylformamide
pka	2.2, 4.4, 6.7(at 25℃)
Colour Index	45350
form	Powder
color	Red to orange
PH Range	Pink uorescence (4.0) to green uorescence (6.0)
Water Solubility	insoluble
ε(extinction coefficient)	≥12000 at 282-286nm
	≥39000 at 236-240nm
	≥70000 at 488-492nm
λmax	493.5nm, 496nm, 460nm, 515nm
Merck	144,159
BRN	94324
Stability:	Stable. Combustible. Incompatible with strong oxidizing agents.
Major Application	Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
Biological Applications	Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
LogP	2.980 (est)
CAS DataBase Reference	2321-07-5(CAS DataBase Reference)
EPA Substance Registry System	Fluorescein (2321-07-5)

Fluorescent whitening agent Textile dye Plastic product colorant

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Chemical detection methods

Spectroscopic Analysis Methods

Ultraviolet-Vis Spectrophotometry (UV-Vis)
Infrared Spectroscopy (IR/FTIR)
Atomic Absorption Spectroscopy (AAS)
X-ray Fluorescence Spectroscopy (XRF)
Nuclear Magnetic Resonance Spectroscopy (NMR)

Chromatographic Analysis

Gas Chromatography (GC)
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Mass Spectrometry Analysis

GC-MS / LC-MS/MS: Qualitative and Quantitative Analysis
ICP-MS: Trace Metal Elements (ppb Level)
TOF-MS: Precise Molecular Weight Determination

Electrochemical analysis methods

Polypotentiometric methods
Conductivity methods
Polarographic methods / Voltammetric methods
Coulometric analysis

Thermal Analysis Methods

Thermogravimetric Analysis (TGA)
Differential Scanning Calorimetry (DSC)
Differential Thermal Analysis (DTA)

Classical wet chemical analysis

Titration analysis
Gravity analysis