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Fluorescein Cas 2321-07-5

Fluorescein appears as orange yellow or light orange red crystalline powder. Melting point 314-316 ℃ (decomposition). Soluble in hot ethanol, glacial acetic acid, and alkaline solutions, exhibiting green fluorescence, insoluble in water, benzene, chloroform, and ether.
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  • 2321-07-5

  • bosschemical

  • 2321-07-5

Fluorescein Basic information
Product Name: Fluorescein
CAS: 2321-07-5
MF: C20H12O5
MW: 332.31
EINECS: 219-031-8
Mol File: 2321-07-5.mol
Fluorescein Chemical Properties
Melting point 320 °C(lit.)
Boiling point 429.44°C (rough estimate)
density 1.2739 (rough estimate)
refractive index 1.5000 (estimate)
storage temp. room temp
solubility Solubility Insoluble in water, ether, benzene, chloroform; soluble in ethanol, methanol, acetone, Iethyl acetate, N,N-dimethylformamide
pka 2.2, 4.4, 6.7(at 25℃)
Colour Index 45350
form Powder
color Red to orange
PH Range Pink uorescence (4.0) to green uorescence (6.0)
Water Solubility insoluble
ε(extinction coefficient) ≥12000 at 282-286nm
≥39000 at 236-240nm
≥70000 at 488-492nm
λmax 493.5nm, 496nm, 460nm, 515nm
Merck 144,159
BRN 94324
Stability: Stable. Combustible. Incompatible with strong oxidizing agents.
Major Application Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
Biological Applications Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
LogP 2.980 (est)
CAS DataBase Reference 2321-07-5(CAS DataBase Reference)
EPA Substance Registry System Fluorescein (2321-07-5)

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