99% Bicine CAS 150-25-4 N,N-bis(2-hydroxyethyl)glycine

Certificate of Analysis-COA 

99% Content

 Applications 

1. Protein & Enzyme Biochemistry — enzyme assays, protein storage buffers, activity studies: A zwitterionic buffer with a pKa around 8.3, covering the pH 7.6–9.0 window where many enzymes are most active and where Tris starts to lose control. Low ionic strength contribution and negligible metal binding interference make it a clean background for kinetic work.

2. Electrophoresis & Separation Science — Tris-Bicine gel systems, running buffers, large-protein and membrane-protein separations: Bicine-based gels resolve high molecular weight and highly hydrophobic proteins that Tris-Glycine systems cannot separate cleanly. The trailing ion chemistry gives sharper bands and better recovery of membrane proteins.

3. Protein Crystallisation — crystallisation screens, precipitant and buffer optimisation: Sits in the buffer set used to screen the mildly alkaline range, where many proteins crystallise and where common buffers like citrate and acetate cannot reach.

5. Metal Chelation & Formulation Stabilisation — chelating buffer in formulations, metal-sensitive systems: The bis-hydroxyethyl arms give it mild chelating ability alongside its buffering action, useful where trace metals destabilise an enzyme or a formulation but a strong chelator such as EDTA would strip essential cofactors.

6. Electrochemical & Industrial pH Control — electrochemical baths, process streams, mildly alkaline buffering: Holds pH in the alkaline range without introducing a volatile amine or a strong mineral acid, and without the temperature sensitivity that troubles Tris-based systems.

 Quality Assurance 

Every batch of chemicals leaves our factory with complete laboratory documentation — not a promise, but verifiable test data. From incoming raw material inspection to finished-goods release, each batch is tested against published specifications, and a Certificate of Analysis (COA) accompanies every single shipment. What you order is exactly what we test — and what we ship.

Tested by our in-house ISO 9001:2015 laboratory. Verified independently by third-party labs on request (SGS / BV / Intertek). Traceable from raw material to your warehouse.

 Packaging 

Liquid plastic drums

Liquid tonnes

Liquid iron drums

Export-grade packaging, custom labels

Solids (woven bags/cardboard drums/ton bags)

Liquids (plastic drums/IBC/ISO tanks)